Identification of a highly reactive threonine residue at the active site of gamma-glutamyl transpeptidase.
نویسندگان
چکیده
منابع مشابه
Gamma-glutamyl transpeptidase in cancer diagnosis.
Serum gamma-glutamyl transpeptidase ( G G T ), alkaline phosphatase (A L P ) and aspartate aminotransferase (A ST) were assayed in patients with cancer. GGT was more sensitive than either ALP or AST in detecting metastatic car cinoma to the liver in both icteric and anicteric patients with cancer. Extremely high activities of GGT were seen in patients with hepatoma, carcinoma of the pancreas a...
متن کاملGamma-glutamyl transpeptidase--its role in hepatocarcinogenesis.
Introduction The enzyme gamma-glutamyl transpeptidase (GGT)* is widely used as a marker in preneoplastic lesions in the liver during chemical carcinogenesis (1). The discovery of a link between GGT and liver carcinogenesis first occurred in 1972 when Fiala and co-workers reported elevated levels of the enzyme in livers of rats fed the hepatocarcinogen 3'-methyl-4dimethylaminoazobenzene and also...
متن کاملA glutamyl residue in the active site of triphosphopyridine nucleotide-dependent isocitrate dehydrogenase of pig heart.
The maximum velocity of the reaction catalyzed by the pig heart TPN-specific isocitrate dehydrogenase depends on the basic form of an enzymatic group of pK 5.7. This pK is independent of temperature from 10-30” and increases in 20% ethanol, suggesting the ionization of a carboxyl group. The enzyme is inactivated by incubation at pH 7.0 with 1 cyclohexyl3 (2 morpholinoethyl) carbodiimide either ...
متن کاملIdentification of a reactive cysteine residue at the glutamine binding site of carbamyl phosphate synthetase.
Carbamyl phosphate synthetase from Escherichia coli, which is composed of a light and a heavy subunit, is inhibited with respect to its glutamine-dependent reactions by treatment with ~-2-amino-4-oxo-5-chloro[5-‘~C]pentanoic acid. This glutamine analog reacts with a glutamineor albizziinprotectable site on the light subunit to yield an enzyme covalently linked to a 4-oxo[W]norvaline moiety. The...
متن کاملAn Electrochemical Method to Detect Gamma Glutamyl Transpeptidase
Gamma glutamyl transpeptidase (GGT) is a transferase, which is of great importance in sustaining intracellular cysteine and glutathione levels. The abnormal expression of GGT is significantly associated with features of many metabolic syndromes (e.g., hepatocellular carcinoma). Therefore, it is essential to develop methods to detect GGT so as to monitor the physiological or pathological phenome...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1990
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.87.5.1706